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the number of users doing crystallization trials using the same set up can be checked by comparing the best hits in databases such as razorpages and myproteins. when you are in the process of adding new chemicals, recording the best hits with your data will help in making a quality decision when choosing which chemical(s) to continue to use. it is not advisable to change chemicals during the growth process. the best hits and the crystallization conditions for a given protein can be checked at various stages of crystallization trials to provide valuable information. as crystals grow larger, hanging drop vapor diffusion becomes more difficult. this is because the amount of drop volume used is less and it is necessary to remove more of the mother liquor before the drop can be diluted. when one is removing the mother liquor before the drop becomes diluted, another important point is to make sure the tip of the pipette is not contaminated and the liquid is not allowed to touch the glass surface. this will promote the crystallization of the protein-detergent complex. one can consider increasing the drop size and the number of drops per well to try to ensure that all protein-detergent complexes are seeded and that the best conditions are tried. if the drop size is small and the number of drops is few (less than ten) then one can expect this to be successful.
go in an examine records for cases where attempts have been made to grow crystals with identical reagent and crystallization conditions. do the best hits have equal score? have the best hits from the same data set the same pdb code? if they dont it is always a good idea to repeat your crystallization experiments with slight variations in the crystallization conditions, reagents, durations, temperatures and with the addition or subtraction of other chemicals. this way you can spot the conditions that are working for you and may stop using the conditions that are not working for you. even before you start experimenting with slight variations in the conditions it is advisable to look at the best hits for cases where attempts have been made to grow crystals with identical reagent and crystallization conditions. if the best hits are not equal then you can consider making a small change in the crystallization conditions that you are trying to make. for example, if you had thes' + in the mother liquor try + - in the mother liquor. 3d9ccd7d82